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Premier Biosoft primer premier version 5.0. software
Primer Premier Version 5.0. Software, supplied by Premier Biosoft, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+premier+software+version+5%2E0/primer+premier+5+0+software/pmc12206813-70-10-15
Average 90 stars, based on 1 article reviews
primer premier version 5.0. software - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Development of a duplex TaqMan real-time RT-PCR assay for simultaneous detection of goose astrovirus genotypes 1 and 2.
Article Snippet: Goose astrovirus (GAstV) is a novel pathogen that was discovered in 2018.. It has two genotypes, GAstV-1 and GAstV-2, and both can cause visceral gout of goslings and result in significant economic losses.. The present work aimed to develop a duplex TaqMan real-time quantitative reverse transcription PCR (RT-qPCR) assay to distinguish the two genotypes.

Article Title: A synonymous mutation in IGF-1 impacts the transcription and translation process of gene expression
Article Snippet: Primers in this study were designed using primer Premier Software version 5.0 (Premier Biosoft International) and synthesized by Jilin Comate Bioscience.

Article Title: Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen
Article Snippet: After a bioinformatics analysis of the DPOL gene of PCMV, specific primers-pair and a TaqMan probe were designed using Primer Premier Software version 5.0 (Premier Biosoft, Palo Alto, CA, USA).

Article Title: A synonymous mutation of rs1137070 cause the mice Maoa gene transcription and translation to decrease
Article Snippet: The design of primers for this study was facilitated by primer Premier Software version 5.0 (Premier Biosoft International) and the synthesized primers were provided by Hunan Accurate Biology Bioscience.

Software:

Article Title: The PLAG1 mRNA expression analysis among genetic variants and relevance to growth traits in Chinese cattle.
Article Snippet: Pleomorphic adenoma gene 1 (PLAG1) encodes a developmentally regulated zinc finger protein, locating in growth-related QTNs.. The mRNA expression of this gene was investigated in different tissues and from two different developmental periods, whilst to explore the functions of PLAG1 in growth traits of cattle.. The results showed that PLAG1 was expressed in all examined tissues.

Article Title: Effect of glycolysis and heat shock proteins on hypoxia adaptation of Tibetan sheep at different altitude.
Article Snippet: Glycolysis and heat shock proteins (HSPs) play an important role in mediating the physiological response to hypoxia.. The changes of glycolysis and HSPs with altitude would provide important information regarding ways to prevent hypoxia-related sickness in both animals and humans.. In this study, the expression pattern of HIF1A, PDK4, HSP27 and HSP60, indexes activity and content of glucose metabolism were detected in heart, lung, brain, and quadriceps femoris taken from Tibetan sheep (Ovis aries) that were raised at different altitudes (2,500 m, 3,500 m and 4,500 m).

Article Title: Epistasis between genetic variations on MdMYB109 and MdHXK1 exerts a large effect on sugar content in apple fruit.
Article Snippet: Many quantitative traits are controlled by multiple genetic variations with minor effects, making it challenging to resolve the underlying genetic network and to apply functional markers in breeding.. Affected by up to a hundred quantitative trait loci (QTLs), fruit-soluble sugar content is one of the most complex quantitative traits in apple (Malus sp.).. Here, QTLs for apple fruit sucrose and fructose content were identified via QTL mapping and bulked-segregant analysis sequencing (BSA-seq) using a population derived from a ‘Jonathan’ 3 ‘Golden Delicious’ cross.

Article Title: Characterization and fine mapping of a lesion mimic mutant (Lm5) with enhanced stripe rust and powdery mildew resistance in bread wheat (Triticum aestivum L.).
Article Snippet: Key message A novel light intensity-dependent lesion mimic mutant with enhanced disease resistance was physiologically, biochemically, and genetically characterized, and the causative gene was fine mapped to a 1.28 Mbp interval containing 17 high-confidence genes.. Abstract Lesion mimic mutants are ideal for studying disease resistance and programmed cell death photosynthesis in plants to improve crop yield.. In this study, a novel light intensity-dependent lesion mimic mutant (MC21) was obtained from the wheat variety Chuannong16 (CN16) by ethyl methane sulfonate treatment.

Sequencing:

Article Title: The PLAG1 mRNA expression analysis among genetic variants and relevance to growth traits in Chinese cattle.
Article Snippet: Pleomorphic adenoma gene 1 (PLAG1) encodes a developmentally regulated zinc finger protein, locating in growth-related QTNs.. The mRNA expression of this gene was investigated in different tissues and from two different developmental periods, whilst to explore the functions of PLAG1 in growth traits of cattle.. The results showed that PLAG1 was expressed in all examined tissues.

Article Title: Epistasis between genetic variations on MdMYB109 and MdHXK1 exerts a large effect on sugar content in apple fruit.
Article Snippet: Many quantitative traits are controlled by multiple genetic variations with minor effects, making it challenging to resolve the underlying genetic network and to apply functional markers in breeding.. Affected by up to a hundred quantitative trait loci (QTLs), fruit-soluble sugar content is one of the most complex quantitative traits in apple (Malus sp.).. Here, QTLs for apple fruit sucrose and fructose content were identified via QTL mapping and bulked-segregant analysis sequencing (BSA-seq) using a population derived from a ‘Jonathan’ 3 ‘Golden Delicious’ cross.

Real-time Polymerase Chain Reaction:

Article Title: Epistasis between genetic variations on MdMYB109 and MdHXK1 exerts a large effect on sugar content in apple fruit.
Article Snippet: Many quantitative traits are controlled by multiple genetic variations with minor effects, making it challenging to resolve the underlying genetic network and to apply functional markers in breeding.. Affected by up to a hundred quantitative trait loci (QTLs), fruit-soluble sugar content is one of the most complex quantitative traits in apple (Malus sp.).. Here, QTLs for apple fruit sucrose and fructose content were identified via QTL mapping and bulked-segregant analysis sequencing (BSA-seq) using a population derived from a ‘Jonathan’ 3 ‘Golden Delicious’ cross.



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